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生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
purified antibody
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
分子量
calculated mol wt 139.13 kDa
observed mol wt ~67 kDa
純化經由
(Unpurified)
物種活性
virus
包裝
antibody small pack of 100 μL
技術
ELISA: suitable
neutralization: suitable
western blot: suitable
表位序列
C-terminal half
Protein ID登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
vaccinia virus ... s> S(43740568)
一般說明
Spike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. (Ref.: Ravichandran, S., et al. (2020). Sci. Transl. Med. 12(550); eabc3539).
特異性
This rabbit polyclonal antibody detects the S2 domain of SARS-CoV-2 spike protein.
免疫原
His-tagged recombinant fragment corresponding to the S2 domain from the extracellular region of SARS-CoV-2 spike protein.
應用
Quality Control Testing
Evaluated by Western Blotting in SARS-CoV-2 spike S2 ECD Recombinant protein.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SARS-CoV-2 spike protein S2 domain in SARS-CoV-2 spike S2 ECD Recombinant protein.
Tested Applications
ELISA Analysis: A representative lot detected SARS-CoV-2 spike protein S2 domain in ELISA applications (Ravichandran, S., et. al. (2020). Sci Transl Med. 12(550):eabc3539).
Neutralizing: A representative lot neutralized SARS-CoV-2 spike protein S2 in Neutralizing applications (Ravichandran, S., et. al. (2020). Sci Transl Med. 12(550):eabc3539).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in SARS-CoV-2 spike S2 ECD Recombinant protein.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SARS-CoV-2 spike protein S2 domain in SARS-CoV-2 spike S2 ECD Recombinant protein.
Tested Applications
ELISA Analysis: A representative lot detected SARS-CoV-2 spike protein S2 domain in ELISA applications (Ravichandran, S., et. al. (2020). Sci Transl Med. 12(550):eabc3539).
Neutralizing: A representative lot neutralized SARS-CoV-2 spike protein S2 in Neutralizing applications (Ravichandran, S., et. al. (2020). Sci Transl Med. 12(550):eabc3539).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-SARS-CoV-2 spike protein S2 domain, Cat. No. ABF1063, is a rabbit polyclonal antibody that detects the S2 domain of SARS-CoV-2 spike protein and is tested for use in ELISA, Neutralization, and Western Blotting.
外觀
Rabbit polyclonal antibody in serum without preservatives.
儲存和穩定性
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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