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Key Documents

17-191

Sigma-Aldrich

MAP Kinase/Erk Assay Kit, non-radioactive

MAP Kinase/Erk Assay Kit is designed to measure the phosphotransferase activity of MAP (mitogen-activated protein) Kinase in immunoprecipitates & column fractions.

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.84

品質等級

製造商/商標名

Upstate®

技術

activity assay: suitable (kinase)

UniProt登錄號

運輸包裝

wet ice

應用

MAP Kinase/Erk Assay Kit is designed to measure the phosphotransferase activity of MAP (mitogen-activated protein) Kinase in immunoprecipitates & column fractions.
Used to detect/quantify: MAP Kinase/Erk

成分

Assay Dilution Buffer I (ADBI) (Cat.# 20-108)

MAP Kinase/Erk Substrate Cocktail II (Cat.# 20-166)

Inhibitor Cocktail (Cat.# 20-116)

Magnesium/ATP Cocktail (Cat.# 20-113)

Anti-phospho-MBP, clone P12 (Cat.# 05-429)

品質

The assay kit is designed to measure the phosphotransferase activity of MAP (mitogen-activated protein) Kinase in immunoprecipitates and column fractions. The assay kit is based on phosphorylation of a specific substrate (myelin basic protein, MBP). The phosphorylated substrate is then analyzed by immunoblot analysis, probing with a monoclonal Phospho-specific MBP antibody.

其他說明

This product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

12 - Non Combustible Liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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K B Andersson et al.
European journal of immunology, 26(12), 3170-3178 (1996-12-01)
CD22 is a B cell-restricted surface molecule which may play an important role in interactions between B cells and other cells and in regulating signals through the B cell receptor (BCR) complex. Here we have examined whether the mouse is
Madhukar Varshney et al.
Analytical chemistry, 80(6), 2141-2148 (2008-02-15)
Nanomechanical resonators have shown potential application for mass sensing and have been used to detect a variety of biomolecules. In this study, a dynamic resonance-based technique was used to detect prion proteins (PrP), which in conformationally altered forms are known
M M Ilondo et al.
Biochemical and biophysical research communications, 134(2), 671-677 (1986-01-29)
Human growth hormone was labelled with 125 Iodine by the stoichiometric modification of the chloramine-T method to a specific activity of 50-80 microCi/microgram, and the iodinated mixture was purified by reverse-phase high performance liquid chromatography using a C18 column (SynChropak
T Hiroi et al.
European journal of immunology, 24(11), 2653-2658 (1994-11-01)
The major purpose of this study was to elucidate Th1 [interferon (IFN)-gamma and interleukin (IL)-2] and Th2 (IL-4, IL-5 and IL-6) cytokine-producing CD3+ T cells in salivary glands, which are the major mucosal effector tissues in the oral region. Thus
Ignazia Tusa et al.
Stem cell reports, 11(4), 929-943 (2018-09-25)
Tyrosine kinase inhibitors (TKi) are effective against chronic myeloid leukemia (CML), but their inefficacy on leukemia stem cells (LSCs) may lead to relapse. To identify new druggable targets alternative to BCR/ABL, we investigated the role of the MEK5/ERK5 pathway in

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