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Merck
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Key Documents

1.04208

Sigma-Aldrich

Brilliant green (hydrogen sulfate) (C.I. 42040)

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About This Item

经验公式(希尔记法):
C27H34N2O4S
CAS号:
分子量:
482.63
MDL號碼:
分類程式碼代碼:
41116130
NACRES:
NA.21

形狀

solid

品質等級

效力

300-400 mg/kg LD50, oral (Rat)

成份

Dye, ≥95% spectrophotometric assay

pH值

2.5 (25 °C, 10 g/L in H2O)

mp

210 °C (decomposition)

溶解度

100 g/L

體積密度

500‑600 kg/m3

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

2-30°C

InChI

1S/C27H33N2.H2O4S/c1-5-28(6-2)25-18-14-23(15-19-25)27(22-12-10-9-11-13-22)24-16-20-26(21-17-24)29(7-3)8-4;1-5(2,3)4/h9-21H,5-8H2,1-4H3;(H2,1,2,3,4)/q+1;/p-1

InChI 密鑰

NNBFNNNWANBMTI-UHFFFAOYSA-M

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一般說明

Brilliant green (hydrogen sulfate) (C.I. 42040) for microscopy Certistain®, is a dry staining dye that is used to prepare a staining solution makes target structures in bacteriological specimen materials evaluable for diagnostic purposes. In the area of bacteriology, among others the Gram staining method, the Ziehl-Neelsen staining method, and the spore-staining method according to Rakette are used to yield a differentiated image of pathogens. This can be done either by using eosin Y as a counterstain or by modification according to Wirtz using safranine O solution. By this method, spores are always stained green and the cell elements always red. As CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications.

應用

for microscopy Certistain®

分析報告

Dye content (spectrophotometrically): ≥ 95 %
Identity
- Hydrogen sulfate (as sulfur):
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 628 - 632 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 2210 - 2350
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

法律資訊

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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