06-543

Anti-FAK Antibody

Upstate^®, from rabbit

• 别名: FADK 1, PTK2 protein tyrosine kinase 2, Protein-tyrosine kinase 2, focal adhesion kinase 1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human, hamster, mouse, rat
• 應無反應活性: avian
• 製造商／商標名: Upstate^®
• 技術: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG
• NCBI登錄號: NM_005607; NM_153831
• UniProt登錄號: Q05397
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PTK2(5747)

说明

一般說明

Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and it′s phosphorylation states have been implicated in cancer metastasis and tumor cell survival and adhesion-independent growth. Additionally, recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention.

免疫原

pGEX-derived fusion protein containing residues 748-1053 of human FAK

應用

Immunocytochemistry: 10 μg/mL of a previous lot showed positive immunostaining for Fak in Ref 52 cells fixed with 3.7% formaldehyde.

Immunoprecipitation: 4 μg of a previous lot immunoprecipitated FAK from 500 μg of murine 3T3/A31 RIPA lysate.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

This Anti-FAK Antibody is validated for use in WB, IP, IC for the detection of FAK.

品質

Evaluated by western blot on RIPA lysates from murine 3T3/A31 or human A431 RIPA cell lysate.

Western Blot Analysis: 0.5-2 μg/mL of this antibody detected FAK in RIPA lysates from murine 3T3/A31 and previously in human A431 RIPA cell lysate.

標靶描述

125 kDa

聯結

Replaces: 04-591

外觀

Protein A purified

Format: Purified

Purified rabbit polyclonal IgG in buffer containing storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide). Store at -20°C.

儲存和穩定性

Stable for 1 year at from date of receipt.

分析報告

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable