05-1403

Anti-Giα2 Antibody, clone L5

clone L5, from mouse

• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: L5, monoclonal
• 物種活性: guinea pig (43%), rat (69%), bovine (100%), human (62%), mouse (69%)
• 技術: immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG2b
• NCBI登錄號: NM_002070.2
• UniProt登錄號: P04899
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... GNAI2(2771)

说明

特異性

Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB.

This antibody recognizes Giα-2.

免疫原

Recombinant Giα-2, purified by the method of Linder (J. Biol. Chem. [1990] 265, 8243).

應用

Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer

Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes

Research Category
Signaling

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

This Anti-Giα2 Antibody, clone L5 is validated for use in WB, IH(P) for the detection of Giα2.

品質

Routinely evaluated by Western Blot on Huvec lysates.

Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates.

標靶描述

Labels a single protein band of 39-42 kDa in bovine and rat membranes.

聯結

Replaces: MAB3077

外觀

Purified

Format: Purified

Purified mouse monoclonal IgG2b, in 0.02 M phosphate buffer, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide.

儲存和穩定性

Maintain at 2-8°C for 1 year from date of receipt.

分析報告

Control
Huvec cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable