Skip to Content
Merck

US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C18

US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C18 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

581322-U

Ascentis® C18 HPLC Column

3 μm particle size, L × I.D. 15 cm × 4.6 mm

581325-U

Ascentis® C18 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

581408-U

Ascentis® C8 HPLC Column

3 μm particle size, L × I.D. 15 cm × 4.6 mm

581425-U

Ascentis® C8 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

53829-U

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

53838-U

Ascentis® Express C8, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

accessory

Product No.
Description
Pricing

59660-U

Supelguard™ Guard Cartridge Holder

Stand-alone, for use with Supelguard cartridges (2 cm L. x 2.1 to 4.0 mm I.D.)

guard cartridge

Product No.
Description
Pricing

581379-U

Ascentis® C18 Supelguard™ Guard Cartridge

3 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea

581426-U

Ascentis® C8 Supelguard Guard Cartridge

5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea

CONDITIONS

column

Ascentis C18, 25 cm x 4.6 mm I.D., 5 μm particles (581325-U)

mobile phase

[A] water; [B] acetonitrile

gradient

10 to 50% B in 13 min; to 60% B in 10 min; to 90% B in 6 min; held for 5 min

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 210 nm

injection

10 μL

sample

as indicated in 16% acetonitrile in water

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS