Calpain mobilizes Atg9/Bif-1 vesicles from Golgi stacks upon autophagy induction by thapsigargin.

Calpain mobilizes Atg9/Bif-1 vesicles from Golgi stacks upon autophagy induction by thapsigargin.

Biology open (2017-03-18)

Elena Marcassa, Marzia Raimondi, Tahira Anwar, Eeva-Liisa Eskelinen, Michael P Myers, Gianluca Triolo, Claudio Schneider, Francesca Demarchi

PMID28302665

ABSTRACT

CAPNS1 is essential for stability and function of the ubiquitous calcium-dependent proteases micro- and milli-calpain. Upon inhibition of the endoplasmic reticulum Ca

MATERIALS

Product Number

Brand

Product Description

T9033

Sigma-Aldrich

Thapsigargin, ≥98% (HPLC), solid film

X100

Sigma-Aldrich

Triton^™ X-100, laboratory grade

F3165

Sigma-Aldrich

Monoclonal ANTI-FLAG^® M2 antibody produced in mouse, clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

C0230

Sigma-Aldrich

Monoclonal Anti-m/μ Calpain, Small Subunit antibody produced in mouse, ~1 mg/mL, clone 28F3, purified immunoglobulin, buffered aqueous glycerol solution

A2066

Sigma-Aldrich

Anti-Actin antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution