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  • Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes.

Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes.

Current opinion in microbiology (2003-07-02)
Michael Wagner, Matthias Horn, Holger Daims
ABSTRACT

Fluorescence in situ hybridisation with rRNA-targeted nucleic acid probes can be used to directly identify microorganisms within complex samples in a few hours and therefore has widespread application in environmental and medical microbiology. The past year has seen significant methodological improvements in fluorescence in situ hybridisation, as well as in the combination of this method with other techniques for inferring functional traits of microorganisms within their environment.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Enterococcus faecium FISH probe – ATTO 488, Probe for fluorescence in situ hybridization (FISH), 20µM in water
Sigma-Aldrich
Eubacteria FISH probe extension set, EUBII-Cy3 and EUBIII-Cy3 FISH (fluorescence in situ hybridization) probes for research, 20 μM in water
Sigma-Aldrich
Bacterial negative control FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water
Sigma-Aldrich
Porphyromonas gingivalis FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water
Sigma-Aldrich
Proteus FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water
Sigma-Aldrich
Eubacteria FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH), 20 µM in water
Sigma-Aldrich
Akkermansia muciniphila FISH probe-ATTO488, Probe for fluorescence in situ hybridization (FISH), 20µM in water
Sigma-Aldrich
Enterococcus faecium FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH), 20µM in water
Sigma-Aldrich
Escherichia coli FISH probe -ATTO488, Probe for fluorescence in situ hybridization (FISH), 20µM in water
Sigma-Aldrich
Bacterial negative control FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH),20 μM in water
Sigma-Aldrich
Eubacteria FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH)
Sigma-Aldrich
Akkermansia muciniphila FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water
Sigma-Aldrich
Escherichia coli FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH)
Sigma-Aldrich
Porphyromonas gingivalis FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH)
Sigma-Aldrich
Proteus FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH),20 μM in water
Sigma-Aldrich
Alphaproteobacteria FISH probe-Cy3, Probe for fluorescence in situ hybridization (FISH), 20 μM in water