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  • Use of exogenous volatile organic compounds to detect Salmonella in milk.

Use of exogenous volatile organic compounds to detect Salmonella in milk.

Analytica chimica acta (2018-06-10)
Najat H O Bahroun, John D Perry, Stephen P Stanforth, John R Dean
ABSTRACT

Rapid, sensitive, and selective detection and identification of pathogenic bacteria is required in terms of food security. In this study, exogenous VOCs liberated by Salmonella strains have been identified and quantified via head space-solid phase microextraction gas chromatography mass spectrometry (HS-SPME-GC-MS) in milk samples. The specific enzymes targeted for detection and/or differentiation of Salmonella were C8 esterase, α-galactosidase and pyrrolidonyl peptidase using the following enzyme substrates: 2-chlorophenyl octanoate, phenyl α-d-galactopyranoside and L-pyrrollidonyl fluoroanilide, respectively. Detection of the exogenous VOCs, 2-chlorophenol, phenol and 3-fluoraniline was possible with typical limits of detection of 0.014, 0.045 and 0.005 μg/mL, respectively and correlation coefficients >0.99. The developed methodology was able to detect and identify Salmonella species within a 5 h incubation at 37 °C by the detection of the liberated VOCs. It was found that the milk samples tested were Salmonella free.

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Supelco
SPME fiber assortment kit 5, needle size 23 ga, for use with autosampler
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Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, for use with autosampler, needle size 23 ga, metal alloy fiber
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Supelco
SPME fiber assortment kit 4, for use with autosampler, size 24 ga
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Supelco
SPME StableFlex fiber assortment kit, needle size 24 ga, for use with autosampler
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Supelco
SPME fiber assortment kit 4, for use with autosampler, needle size 23 ga
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Supelco
SPME fiber assortment kit 2, for use with autosampler, needle size 23 ga
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Supelco
SPME fiber assortment kit 1, for use with autosampler, needle size 23 ga
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Supelco
SPME StableFlex fiber assortment kit, needle size 23 ga, for use with autosampler
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Supelco
SPME fiber assortment kit 2, for use with autosampler, size 24 ga
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Supelco
SPME fiber assortment kit 4, for use with manual holder, size 24 ga
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Supelco
SPME fiber assortment kit 2, for use with manual holder, needle size 24 ga
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Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, for use with autosampler
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Supelco
SPME StableFlex fiber assortment kit, for use with manual holder
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Supelco
SPME fiber assortment kit 1, for use with autosampler, needle size 24 ga
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Supelco
SPME fiber assortment kit 1, for use with manual holder, needle size 24 ga
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Supelco
SPME-OC Fiber Assembly, Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 75 μm(Coating thickness includes 65 μm coating + 10 μm OC (overcoating), needle size 23 ga, pkg of 3 ea, fiber L 1 cm
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Sigma-Aldrich
L-Pyroglutamic acid, ≥99.0% (T)
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Sigma-Aldrich
Hexyl octanoate, ≥97%, FG
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Sigma-Aldrich
Octanoyl chloride, 99%
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Supelco
SPME fiber assembly polyacrylate (PA), df 85 μm(PA, for use with autosampler, needle size 23 ga
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Supelco
SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 23 ga, StableFlex, for use with autosampler
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Supelco
SPME fiber assembly, Carbowax-Polyethylene Glycol (PEG) Coating, needle size 23 ga, df 60 μm(PEG, metal alloy fiber, for use with manual holder
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Supelco
SPME fiber assembly, Carbowax-Polyethylene Glycol (PEG) Coating, needle size 23 ga, df 60 μm(PEG, for use with autosampler
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Supelco
SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), for use with autosampler, needle size 23 ga, metal alloy fiber, fiber L 2 cm
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Supelco
SPME fiber assembly Polydimethylsiloxane (PDMS), df 7 μm(PDMS, needle size 23 ga, for use with autosampler
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Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 23 ga, PDMS/DVB StableFlex, for use with autosampler
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Supelco
SPME fiber assembly Polydimethylsiloxane (PDMS), df 30 μm(PDMS, needle size 24 ga, for use with autosampler
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Supelco
SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), df 75 μm(CAR/PDMS, needle size 23 ga, for use with manual holder
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Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, for use with autosampler, needle size 23 ga
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Supelco
SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 24 ga, StableFlex, for use with autosampler
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