ZMS1118
Anti-TOMM20 Antibody, clone 2F8.1 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Mitochondrial import receptor subunit TOM20 homolog;Mitochondrial 20 kDa outer membrane protein;Outer mitochondrial membrane receptor Tom20
Sign Into View Organizational & Contract Pricing
All Photos(20)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
biological source
mouse
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2F8.1, recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 16.30 kDa
observed mol wt ~15 kDa
purified by
using protein G
species reactivity
rat, human, mouse
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... TOMM20(9804)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 2F8.1 is a ZooMAb® Mouse recombinant monoclonal antibody that specifically detects TOMM20.
Immunogen
GST-tagged full-length recombinant human TOMM20.
Application
Quality Control Testing
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TOMM20 in A431 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TOMM20 in HeLa, NIH3T3, and PC12 cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TOMM20 in HeLa, HepG2, and NIH3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TOMM20 in human cerebral cortex and human colon tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TOMM20 in A431 cell lysate.
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TOMM20 in A431 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TOMM20 in HeLa, NIH3T3, and PC12 cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TOMM20 in HeLa, HepG2, and NIH3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TOMM20 in human cerebral cortex and human colon tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TOMM20 in A431 cell lysate.
Target description
Mitochondrial import receptor subunit TOM20 homolog (UniProt: Q15388; also known as Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20) is encoded by the TOMM20 (also known as KIAA0016) gene (Gene ID: 9804) in human. TOMM20 is a single-pass mitochondrial outer protein that is a component of the receptor complex responsible for the recognition and translocation of nuclear-encoded and cytosolically synthesized mitochondrial preproteins. Together with TOM22 it functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Overexpression of TOMM20 has been reported tin various cancers and its expression is correlated with increased mitochondrial mass. Suppression of TOMM20 is shown to reduce proliferation, migration, and invasion in colorectal cancer cells and cause S-phase arrest and apoptosis. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Park, SH., et al. (2019). BMB Rep. 52(12): 712-717).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service