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Key Documents

SRP0707

Sigma-Aldrich

PAD Cocktail, Active

recombinant

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

recombinant

expressed in baculovirus infected Sf9 cells

Assay

70-95% (SDS-PAGE)

form

frozen liquid

mol wt

~95-100 kDa

concentration

0.5 μg/μL (0.1 ug/uL each PAD)

accession no.

BC025718
BC049947
NM_013358
NM_016233
NM_207421

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PADI1(29943) , PADI2(11240) , PADI3(51702) , PADI4(23569) , PADI6(353238)

Application

Useful for citrullination and Western blot.

Biochem/physiol Actions

The five full length recombinant PAD proteins (1, 2, 3, 4 and 6) expressed by baculovirus in Sf9 insect cells using N-terminal GST tags. This mixture is ideally suited for in vitro citrullination of protein targets.

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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