GE17-0948-01
Blue Sepharose™ 6 Fast Flow
Cytiva 17-0948-01, pack of 50 mL
About This Item
Recommended Products
ligand
Cibacron Blue
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 50 mL
manufacturer/tradename
Cytiva 17-0948-01
storage condition
(20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0)
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
3-13
working range
4-12
suitability
suitable for bioprocess medium
storage temp.
2-8°C
Related Categories
General description
Blue Sepharose™ 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.
Blue Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with Cibacron Blue 3G covalently attached by the triazine coupling method.
The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.
Blue Sepharose™ 6 Fast Flow has been developed and tested in co-operation with leading large scale manufacturers and is used in routine commercial production.
Blue Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Blue Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Designed for large-scale purification of interferon and albumin. Also used for isolating groups of enzymes and removing of albumin
- BioProcess medium supported for industrial applications and well-established in approved processes
- Cibacron Blue 3G covalently coupled to the base matrix allowing for low ligand leakage and high capacity
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocols
This page shows how to remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from GE Healthcare.
This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.
Performing a Purification of NAD+-dependent dehydrogenases and ATP-dependent kinases with 5’ AMP Sepharose 4B, HiTrap Blue HP and Blue Sepharose 6 Fast Flow
This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from GE Healthcare.
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