GE17-0920-07
IMAC Sepharose™ High Performance
Cytiva 17-0920-07, pack of 100 mL
About This Item
Recommended Products
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-0920-07
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning in place
2-14(Medium uncharged with metal ions.)
working range
3-12
capacity
≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
General description
Application
Features and Benefits
- For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion
- Conveniently charge with your metal of choice
- 34 μm bead size for high performance purification
- High binding capacity
Storage and Stability
Analysis Note
Legal Information
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from GE Healthcare.
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.
For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
Protocols
Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance
This page shows how to perform a separation with poly (His) fusion proteins which are affinity chromatography tags used in His MicroSpin Purification Module, HisTrap Kit, HiTrap Chelating HP and Chelating Sepharose Fast Flow products from GE Healthcare.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
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