Nutrient Mixture F-12 Ham Formulation

• Nutrient Mixture F12 Ham
• Nutrient Mixture F12 Ham HEPES Modification
• Nutrient Mixture F12 Ham Kaighn's Modification

Nutrient Mixture F-12 Ham

Component	N4888 [1x] g/L	N6658 [1x] g/L	N6760 g/L
Inorganic Salts
Calcium Chloride	0.0333	0.0333	0.0333
Cupric Sulfate • 5H2O	0.0000025	0.0000025	0.0000025
Ferrous Sulfate • 7H2O	0.000834	0.000834	0.000834
Magnesium Chloride	0.0576	0.0576	0.0576
Potassium Chloride	0.224	0.224	0.224
Sodium Bicarbonate	1.176	1.176	—
Sodium Chloride	7.599	7.599	7.599
Sodium Phosphate Dibasic (anhydrous)	0.14204	0.14204	0.14204
Zinc Sulfate • 7H2O	0.000863	0.000863	0.000863
Amino Acids
L-Alanine	0.009	0.009	0.009
L-Arginine • HCl	0.211	0.211	0.211
L-Asparagine • H2O	0.01501	0.01501	0.01501
L-Aspartic Acid	0.0133	0.0133	0.0133
L-Cysteine • HCl • H2O	0.035	0.035	0.035
L-Glutamic Acid	0.0147	0.0147	0.0147
L-Glutamine	—	0.146	0.146
Glycine	0.00751	0.00751	0.00751
L-Histidine • 3HCl • H2O	0.02096	0.02096	0.02096
L-Isoleucine	0.00394	0.00394	0.00394
L-Leucine	0.0131	0.0131	0.0131
L-Lysine • HCl	0.0365	0.0365	0.0365
L-Methionine	0.00448	0.00448	0.00448
L-Phenylalanine	0.00496	0.00496	0.00496
L-Proline	0.0345	0.0345	0.345
L-Serine	0.0105	0.0105	0.0105
L-Threonine	0.0119	0.0119	0.0119
L-Tryptophan	0.00204	0.00204	0.00204
L-Tyrosine • 2Na • 2H2O	0.00778	0.00778	0.00778
L-Valine	0.0117	0.0117	0.0117
Vitamins
D-Biotin	0.0000073	0.0000073	0.000073
Choline Chloride	0.01396	0.01396	0.01396
Folic Acid	0.00132	0.00132	0.00132
myo-Inositol	0.018	0.018	0.018
Niacinamide	0.000037	0.000037	0.000037
D-Pantothenic Acid (hemicalcium)	0.00048	0.00048	0.00048
Pyridoxine • HCl	0.000062	0.000062	0.000062
Riboflavin	0.000038	0.000038	0.000038
Thiamine • HCl	0.00034	0.00034	0.00034
Vitamin B12	0.00136	0.00136	0.00136
Other
D-Glucose	1.802	1.802	1.802
Hypoxanthine	0.00408	0.00408	0.00408
Linoleic Acid	0.000084	0.000084	0.000084
Phenol Red • Na	0.0013	0.0013	0.0013
Putrescine • HCl	0.000161	0.000161	0.000161
Pyruvic Acid • Na	0.11	0.11	0.11
Thioctic Acid	0.00021	0.00021	0.00021
Thymidine	0.00073	0.00073	0.00073
Add
L-Glutamine	0.146	—	—
Sodium Bicarbonate	—	—	1.176

Nutrient Mixture F-12 Ham HEPES Modification

Component	N8641 [1x] g/L
Inorganic Salts
Calcium Chloride	0.0333
Cupric Sulfate • 5H2O	0.0000025
Ferrous Sulfate • 7H2O	0.000834
Magnesium Chloride	0.0576
Potassium Chloride	0.224
Sodium Bicarbonate	1.176
Sodium Chloride	7.1
Sodium Phosphate Dibasic (anhydrous)	0.14204
Zinc Sulfate • 7H2O	0.000863
Amino Acids
L-Alanine	0.009
L-Arginine • HCl	0.211
L-Asparagine • H2O	0.01501
L-Aspartic Acid	0.0133
L-Cysteine • HCl • H2O	0.035
L-Glutamic Acid	0.0147
Glycine	0.00751
L-Histidine • 3HCl • H2O	0.02096
L-Isoleucine	0.00394
L-Leucine	0.0131
L-Lysine • HCl	0.0365
L-Methionine	0.00448
L-Phenylalanine	0.00496
L-Proline	0.0345
L-Serine	0.0105
L-Threonine	0.0119
L-Tryptophan	0.00204
L-Tyrosine • 2Na • 2H2O	0.00778
L-Valine	0.0117
Vitamins
D-Biotin	0.0000073
Choline Chloride	0.01396
Folic Acid	0.00132
myo-Inositol	0.018
Niacinamide	0.000037
D-Pantothenic Acid (hemicalcium)	0.000238
Pyridoxine • HCl	0.000062
Riboflavin	0.000038
Thiamine • HCl	0.00034
Vitamin B12	0.00136
Other
D-Glucose	1.802
HEPES	5.958
Hypoxanthine	0.00408
Linoleic Acid	0.000084
Phenol Red • Na	0.0013
Putrescine • HCl	1.000161
Pyruvic Acid • Na	0.11
Thioctic Acid	0.00021
Thymidine	0.00073
Add
L-Glutamine	0.146

Nutrient Mixture F-12 Ham Kaighn's Modification

Component	N3520 [1x] g/L
Inorganic Salts
Calcium Chloride	0.10207
Cupric Sulfate • 5H2O	0.0000025
Ferrous Sulfate • 7H2O	0.000834
Magnesium Chloride	0.04961
Magnesium Sulfate	0.19264
Potassium Chloride	0.28329
Potassium Phosphate Monobasic	0.058523
Sodium Phosphate Dibasic	0.11502
Sodium Chloride	7.5972
Zinc Sulfate • 7H2O	0.00014375
Amino Acids
L-Alanine	0.017818
L-Arginine	0.4214
L-Asparagine • H2O	0.03002
L-Aspartic Acid	0.02662
L-Cysteine • HCl • H2O	0.07024
L-Glutamic Acid	0.02942
L-Glutamine	0.2922
Glycine	0.015014
L-Histidine • HCl • H2O	0.04192
L-Isoleucine	0.007872
L-Leucine	0.02624
L-Lysine • HCl	0.07304
L-Methionine	0.008952
L-Phenylalanine	0.009912
L-Proline	0.06906
L-Serine	0.02102
L-Threonine	0.02382
L-Tryptophan	0.004084
L-Tyrosine	0.010872
L-Valine	0.02342
Vitamins
D-Biotin	0.00007329
Choline Chloride	0.01396
Folic Acid	0.0013242
Hypoxanthine	0.004083
myo-Inositol	0.01802
Niacinamide	0.00003663
D-Pantothenic Acid (hemicalcium)	0.000477
Putrescine • 2HCl	0.0003222
Pyridoxine • HCl	0.00006168
Riboflavin	0.00003764
Thiamine • HCl	0.0003373
Thymidine	0.0007266
Vitamin B12	0.0013554
Other
D+-Glucose	1.26
Phenol Red • Na	0.00331806
Pyruvic Acid • Na	0.22
DL-6,8-Thioctic Acid	0.0002063
Add
Sodium Bicarbonate	2.5