PIK3CA

PIK3CA

Oncotarget (2017-12-22)

Matthias A Roelli, Dorothée Ruffieux-Daidié, Amandine Stooss, Oussama ElMokh, Wayne A Phillips, Matthias S Dettmer, Roch-Philippe Charles

PMID29262556

ABSTRACT

Thyroid carcinomas are the most prevalent endocrine cancers. The BRAF

MATERIALS

Product Number

Brand

Product Description

320331

Sigma-Aldrich

Hydrochloric acid, ACS reagent, 37%

47036

Sigma-Aldrich

Saponin, for molecular biology, used as non-ionic surfactant

E4378

Sigma-Aldrich

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid, ≥97.0%

P9416

Sigma-Aldrich

TWEEN^® 20, for molecular biology, viscous liquid

C8661

Sigma-Aldrich

Cobalt(II) chloride hexahydrate, BioReagent, suitable for cell culture, suitable for insect cell culture

H7509

Sigma-Aldrich

(Hydroxypropyl)methyl cellulose, viscosity 2,600-5,600 cP, 2 % in H₂O(20 °C)(lit.)

S4641

Sigma-Aldrich

Sodium citrate tribasic dihydrate, ACS reagent, ≥99.0%

HT501128

Sigma-Aldrich

Formalin solution, neutral buffered, 10%, histological tissue fixative

T1503

Sigma-Aldrich

Trizma^® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline

A7906

Sigma-Aldrich

Bovine Serum Albumin, heat shock fraction, pH 7, ≥98%

HT110132

Sigma-Aldrich

Eosin Y Solution, Alcoholic

GHS132

Sigma-Aldrich

Hematoxylin Solution, Gill No. 1

P4780

Sigma-Aldrich

TWEEN^® 80, suitable for cell culture, suitable for insect cell culture, viscous liquid

P6744

Sigma-Aldrich

Picric acid solution, 1.3% in H₂O (saturated)

12322

Sigma-Aldrich

Iron(III) chloride solution, purum, 45% FeCl₃ basis

P2308

Sigma-Aldrich

Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

C0250

Sigma-Aldrich

Sodium cacodylate trihydrate, ≥98%

HT152

Sigma-Aldrich

Phosphotungstic Acid Solution, 10 % (w/v)

F8129

Sigma-Aldrich

Acid Fuchsin, used in tissue staining