- Electrochemical glucose biosensor based on nickel oxide nanoparticle-modified carbon paste electrode.
Electrochemical glucose biosensor based on nickel oxide nanoparticle-modified carbon paste electrode.
Artificial cells, nanomedicine, and biotechnology (2013-06-26)
Ceren Erdem, Derya Koyuncu Zeybek, Gözde Aydoğdu, Bülent Zeybek, Sule Pekyardımcı, Esma Kılıç
PMID23795722
ABSTRACT
In the present work, we designed an amperometric glucose biosensor based on nickel oxide nanoparticles (NiONPs)-modified carbon paste electrode. The biosensor was prepared by incorporation of glucose oxidase and NiONPs into a carbon paste matrix. It showed good analytical performances such as high sensitivity (367 μA mmolL(-1)) and a wide linear response from 1.9×10(-3) mmolL(-1) to 15.0 mmolL(-1) with a limit of detection (0.11 μmolL(-1)). The biosensor was used for the determination of glucose in human serum samples. The results illustrate that NiONPs have enormous potential in the construction of biosensor for determination of glucose.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
Sigma-Aldrich
L-Ascorbic acid, puriss. p.a., ACS reagent, reag. ISO, Ph. Eur., 99.7-100.5% (oxidimetric)
Sigma-Aldrich
Sodium phosphate monobasic dihydrate, 98-100.5% (calc. to the dried substance), meets analytical specification of Ph. Eur., BP, USP, FCC, E 339
Supelco
L-Ascorbic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland