The uptake of ZnO and CuO nanoparticles in the water-flea Daphnia magna under acute exposure scenarios.

The uptake of ZnO and CuO nanoparticles in the water-flea Daphnia magna under acute exposure scenarios.

Environmental pollution (Barking, Essex : 1987) (2014-08-12)

Nathalie Adam, Frédéric Leroux, Dries Knapen, Sara Bals, Ronny Blust

ABSTRACT

In this study the uptake of ZnO and CuO nanoparticles by Daphnia magna was tested. Daphnids were exposed during 48 h to acute concentrations of the nanoparticles and corresponding metal salts. The Daphnia zinc and copper concentration was measured and the nanoparticles were localized using electron microscopy. The aggregation and dissolution in the medium was characterized. A fast dissolution of ZnO in the medium was observed, while most CuO formed large aggregates and only a small fraction dissolved. The Daphnia zinc concentration was comparable for the nanoparticles and salts. Contrarily, a much higher Daphnia copper concentration was observed in the CuO exposure, compared to the copper salt. CuO nanoparticles adsorbed onto the carapace and occurred in the gut but did not internalize in the tissues. The combined dissolution and uptake results indicate that the toxicity of both nanoparticle types was caused by metal ions dissolved from the particles in the medium.

MATERIALS

Product Number

Brand

Product Description

311405

Sigma-Aldrich

Copper, ACS reagent, granular, 10-40 mesh, ≥99.90%

223409

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Copper, shot, −3-+14 mesh, 99%

203122

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357456

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349178

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Copper, foil, thickness 0.25 mm, 99.98% trace metals basis

326445

Sigma-Aldrich

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266086

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IRMM522C

Copper, IRMM^®, certified reference material, 0.5 mm wire

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GF42838162

Copper, tube, 100mm, outside diameter 6.0mm, inside diameter 4mm, wall thickness 1.0mm, as drawn, 99.9%

GF53102438

Copper, tube, 500mm, outside diameter 1.2mm, inside diameter 0.56mm, wall thickness 0.32mm, as drawn, 99.9%

GF31266121

Copper, tube, 200mm, outside diameter 1.5mm, inside diameter 0.6mm, wall thickness 0.45mm, as drawn, 99.9%

GF52673250

Copper, tube, 200mm, outside diameter 1.75mm, inside diameter 0.33mm, wall thickness 0.71mm, as drawn, 99.9%

GF29520963

Copper, tube, 200mm, outside diameter 25.4mm, inside diameter 18.9mm, wall thickness 3.25mm, as drawn, 99.9%

GF37574523

Copper, tube, 200mm, outside diameter 6.35mm, inside diameter 4.93mm, wall thickness 0.71mm, as drawn, 99.9%

GF35241892

Copper, tube, 200mm, outside diameter 0.25mm, inside diameter 0.05mm, wall thickness 0.1mm, as drawn, 99.9%

GF73652250

Copper, tube, 500mm, outside diameter 1.5mm, inside diameter 0.6mm, wall thickness 0.45mm, as drawn, 99.9%

GF35866761

Copper, tube, 200mm, outside diameter 10.4mm, inside diameter 10mm, wall thickness 0.2mm, as drawn, 99.9%

GF82636162

Copper, tube, 100mm, outside diameter 25.4mm, inside diameter 18.9mm, wall thickness 3.25mm, as drawn, 99.9%

GF95128218

Copper - O.F.H.C., foil, 10mm disks, thickness 0.25mm, half hard, 99.95+%

GF42325680

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GF80910323

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