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  • Fas mutation reduces obesity by increasing IL-4 and IL-10 expression and promoting white adipose tissue browning.

Fas mutation reduces obesity by increasing IL-4 and IL-10 expression and promoting white adipose tissue browning.

Scientific reports (2020-07-21)
Eun Wha Choi, Minjae Lee, Ji Woo Song, Kyeongdae Kim, Jungmin Lee, Jehoon Yang, Seo Hyun Lee, Il Yong Kim, Jae-Hoon Choi, Je Kyung Seong
ABSTRACT

Brown adipose tissue generates heat via the mitochondrial uncoupling protein UCP1 to protect against obesity and hypothermia. Fas mutant MRL/lpr mice exhibit a significantly leaner phenotype compared to wild type MRL/MpJ mice. In this study, we evaluated the inflammatory cell population in the adipose tissue of MRL/lpr mice, which could potentially influence their lean phenotype. Furthermore, we compared beige fat activity between the MRL/MpJ and MRL/lpr mice. Fas mutation resulted in high body temperature, improved glucose tolerance, and decreased fat mass and adipocyte size. Fas mutation prevented high-fat diet-induced obesity and decreased the white adipose tissue M1:M2 ratio. When mice were fed a high-fat diet, UCP1, IL-4, IL-10, and tyrosine hydroxylase genes had significantly higher expression in Fas-mutant mice than in wild type mice. After a cold challenge, UCP1 expression and browning were also significantly higher in the Fas-mutant mice. In summary, Fas-mutant mice are resistant to high-fat diet-induced obesity due to increased IL-4 and IL-10 levels and the promotion of thermogenic protein activity and browning in their adipose tissues. STAT6 activation might contribute to M2 polarisation by increasing IL-4 and IL-10 levels while increases in M2 and tyrosine hydroxylase levels promote browning in response to Fas mutation.

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Collagenase from Clostridium histolyticum, suitable for release of physiologically active rat epididymal adipocytes, Type II, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid
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MILLIPLEX® Mouse Adiponectin Magnetic Bead- Single Plex - Endocrine Assay, This MILLIPLEX MAP Mouse Adiponectin Magnetic Bead Single Plex Kit is an Endocrine single plex assay. The analyte available for this kit is Adiponectin.
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