H4652
HMG-1 human
lyophilized powder, ≥90% (SDS-PAGE), Histidine-tagged, recombinant, expressed in E. coli
Synonym(s):
Amphoterin, HMGB1, High-Mobility-Group protein
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About This Item
Recommended Products
recombinant
expressed in E. coli
Quality Level
Assay
≥90% (SDS-PAGE)
form
lyophilized powder
mol wt
25 kDa (215 amino acids; comprises two homologous HMG boxes, rich in basic amino acids, and an acidic tail at the carboxy-terminus)
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
SMILES string
COC(=O)CCCCCCCCCCCCCCO
Gene Information
human ... HMGB1(3146)
Related Categories
General description
HMG-1 (high mobility group protein) is a nuclear protein of molecular weight 30kDa. It is secreted from macrophages, monocytes and necrotic cells. It is ubiquitously expressed. The protein has basic HMG (high mobility group) box domains and an acidic carboxyl-terminus.
Application
HMG-1 (high-mobility-group protein) human has been used:
- to prepare a standard curve for measuring HMG1 using ELISA (enzyme-linked immunosorbent assay) test
- to study the apoptotic ratios of Lewis cells in presence of exogenous HMG-1
- to study the changes in Th (T helper)-17 cell expansion in the presence of HMG-1 under in vitro conditions
- for the migration assay of GM7373 cells
Biochem/physiol Actions
HMG-1 (high mobility group protein) is a non-histone chromosomal protein, which promotes the sequence specific DNA attachment of proteins. HMG1 binds DNA in a non-sequence specific manner. It is involved in DNA replication, transcription and repair. In addition, it is up-regulated in cancers and is associated with cellular growth, vasculogenesis and apoptosis regulation. In the presence of infection or injury, HMG1 is secreted and mediates inflammation. It is associated with the pathogenesis of autoimmune diseases, such as rheumatoid arthritis (RA), inflammatory bowel disease (IBD) and systemic lupus erythematosus (SLE).
Physical form
Lyophilized powder containing HEPES buffer salts, pH 7.8, NaCl, EDTA, Triton X-100, Leupeptin and AEBSF.
Analysis Note
Tested in DNA retardation assay
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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