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A2418

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL number:
MFCD00162640
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

technique(s)

direct ELISA: 1:15,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil tissue
western blot (chemiluminescent): 1:40,000-1:80,000 using deecting β-Actin in total cell extract of HeLa cells (5-10 μg/mL)

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3.

Immunogen

purified mouse IgG

Application

Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in rabbit has been used in :
  • direct enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunohistochemistry

Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.

Biochem/physiol Actions

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG antibody participates in complement fixation and opsonization.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Guangyi Qin et al.
Oncology letters, 15(6), 8756-8760 (2018-05-29)
The present study aimed to investigate the effect of allyl isothiocyanate (AITC) on the viability and apoptosis of the human cervical cancer HeLa cell line in vitro, and to explore the potential underlying mechanisms of this. HeLa cells were treated
S Mohan et al.
Parasitology, 123(Pt 3), 271-276 (2001-10-02)
Pasteuria penetrans is a Gram-positive endospore-producing bacterium that is a parasite of root-knot nematodes. Attachment of endospores to the cuticle of the nematode is the first stage in the infection process. Western blot analysis with monoclonal and polyclonal antibodies that
Sarcoplasmatic and myofibrillar protein changes caused by acute heat stress in broiler chicken
Santos C, et al.
Scientia Agricola, 65(5), 453-458 (2008)
C M Niemeyer et al.
Nucleic acids research, 22(25), 5530-5539 (1994-12-25)
Modified biomolecules were used for the non-covalent assembly of novel bioconjugates. Hybrid molecules were synthesized from short single-stranded DNA and streptavidin by chemical methods using a heterobispecific crosslinker. The covalent attachment of an oligonucleotide moiety to streptavidin provides a specific
Structure and function of human and murine receptors for IgG
Unkeless J C, et al.
Annual Review of Immunology, 6(1), 251-281 (1988)
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