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70188-M

Sigma-Aldrich

pSTBlue-1 Blunt Vector (linearized) - Novagen

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About This Item

UNSPSC Code:
41106610

form

liquid

Quality Level

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

storage temp.

−70°C

General description

The pSTBlue-1 vector is a multi-purpose cloning vector featuring a versatile multiple cloning region, blue/white screening, dual opposed T7/SP6 promoters and dual kanamycin/ampicillin resistance. Restriction sites producing 4-base 3′ overhangs are conveniently positioned near each end of the polylinker to facilitate the generation of unidirectional deletions using exonuclease III.

For enhanced convenience, please consider Introductory pSTBlue-1 Perfectly Blunt®Cloning Kit, or pT7Blue-1 Perfectly Blunt Cloning Kit.

Components

20 rxn40 rxn

•2 × 0.5 µg4 × 0.5 µgBlunt Vector

•10 µl10 µlPositive Control Insert

•100 µl2 × 100 µlEnd Conversion Mix

Warning

Toxicity: Standard Handling (A)

Other Notes

Novy, R.E., et al. 1996. inNovations6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques20, 1004. Magnuson, V.L., et al. 1996. BioTechniques21, 700. Hu, G. 1993. DNA and Cell Biology12, 763.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PT7BLUE is a trademark of Merck KGaA, Darmstadt, Germany
Perfectly Blunt is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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