70188-M
pSTBlue-1 Blunt Vector (linearized) - Novagen
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About This Item
Recommended Products
form
liquid
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
storage temp.
−70°C
General description
The pSTBlue-1 vector is a multi-purpose cloning vector featuring a versatile multiple cloning region, blue/white screening, dual opposed T7/SP6 promoters and dual kanamycin/ampicillin resistance. Restriction sites producing 4-base 3′ overhangs are conveniently positioned near each end of the polylinker to facilitate the generation of unidirectional deletions using exonuclease III.
For enhanced convenience, please consider Introductory pSTBlue-1 Perfectly Blunt®Cloning Kit, or pT7Blue™-1 Perfectly Blunt Cloning Kit.
For enhanced convenience, please consider Introductory pSTBlue-1 Perfectly Blunt®Cloning Kit, or pT7Blue™-1 Perfectly Blunt Cloning Kit.
Components
20 rxn40 rxn
•2 × 0.5 µg4 × 0.5 µgBlunt Vector
•10 µl10 µlPositive Control Insert
•100 µl2 × 100 µlEnd Conversion Mix
•2 × 0.5 µg4 × 0.5 µgBlunt Vector
•10 µl10 µlPositive Control Insert
•100 µl2 × 100 µlEnd Conversion Mix
Warning
Toxicity: Standard Handling (A)
Other Notes
Novy, R.E., et al. 1996. inNovations6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques20, 1004. Magnuson, V.L., et al. 1996. BioTechniques21, 700. Hu, G. 1993. DNA and Cell Biology12, 763.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PT7BLUE is a trademark of Merck KGaA, Darmstadt, Germany
Perfectly Blunt is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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