33-125
PIP5-Kinase HTRF Assay
The PIP5 Kinase HTRF Assay kit provides a method for assaying PI(4)P-5 kinases in a homogenous 384 well format. Suitable for screening of potential inhibitors of these enzymes.
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
Quality Level
species reactivity
mouse, human
manufacturer/tradename
HTRF®
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
detection method
fluorometric (HTRF)
shipped in
dry ice
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Oncology
Oncology
The PIP5 Kinase HTRF Assay kit provides a method for assaying PI(4)P-5 kinases in a homogenous 384 well format. Suitable for screening of potential inhibitors of these enzymes.
Used to detect/quantify: PIP5 Kinase
Biochem/physiol Actions
Target Sub-Family: PI3K/PI4K
Packaging
Kit capacity: 384 wells
Components
Reaction Buffer (4X)
DM A Detection Reagent
DM C Detection Reagent
DM E Detection Reagent
Phosphatidylinositol 4-phosphate (1mM)
Phosphatidylserine (1mM)
Stop Reagent D
DM A Detection Reagent
DM C Detection Reagent
DM E Detection Reagent
Phosphatidylinositol 4-phosphate (1mM)
Phosphatidylserine (1mM)
Stop Reagent D
Storage and Stability
12 months at -70°C
Legal Information
HTRF is a registered trademark of Cis Bio International
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
The Journal of biological chemistry, 292(24), 10239-10249 (2017-05-02)
SIRT5 is a lysine desuccinylase known to regulate mitochondrial fatty acid oxidation and the urea cycle. Here, SIRT5 was observed to bind to cardiolipin via an amphipathic helix on its N terminus. In vitro, succinyl-CoA was used to succinylate liver
Nature protocols, 7(6), 1235-1246 (2012-06-02)
The assessment of mitochondrial respiratory chain (RC) enzymatic activities is essential for investigating mitochondrial function in several situations, including mitochondrial disorders, diabetes, cancer, aging and neurodegeneration, as well as for many toxicological assays. Muscle is the most commonly analyzed tissue
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service