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biological source
bovine
Quality Level
form
liquid
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
General description
MBP, Dephosphorylated is made from MBP (catalog #13-104) which is basally phosphorylated and treated with lambda protein phosphatase (catalog #14-405) for complete dephosphorylation.
Application
Myelin basic protein purified from bovine brain and de-phosphorylated using Lambda protein phosphatase. No detectable endogenous phosphorylation as determined by immunoblotting 1 microgram of MBP with 1 microgram/ml anti phospho MBP.
Quality
routinely evaluated as a substrate in a kinase assay using MAP Kinase 2/Erk2 (Catalog # 14-173)
Physical form
10mM MOPS, pH 7.0, 128mM MnCl2, 641mM EDTA, 1.134μg inactive lambda phosphatase and 0.05% sodium azide
Storage and Stability
2 years at -20°C
Other Notes
This product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Gaëlle Lentini et al.
Nature communications, 12(1), 3788-3788 (2021-06-20)
Active host cell invasion by the obligate intracellular apicomplexan parasites relies on the formation of a moving junction, which connects parasite and host cell plasma membranes during entry. Invading Toxoplasma gondii tachyzoites secrete their rhoptry content and insert a complex
J W Crabb et al.
Protein science : a publication of the Protein Society, 7(3), 746-757 (1998-04-16)
Cellular retinaldehyde-binding protein (CRALBP) is abundant in the retinal pigment epithelium (RPE) and Müller cells of the retina where it is thought to function in retinoid metabolism and visual pigment regeneration. The protein carries 11-cis-retinal and/or 11-cis-retinol as endogenous ligands
Thrombospondin stimulates focal adhesion disassembly through Gi- and phosphoinositide 3-kinase-dependent ERK activation
Orr, A. W., et al
The Journal of Biological Chemistry, 277, 20453-20460 (2002)
Cyclic AMP decreases the phosphorylation state of myelin basic proteins in rat brain cell cultures.
Ulmer, J B, et al.
The Journal of Biological Chemistry, 262, 1748-1755 (1987)
D L Lefebvre et al.
The Biochemical journal, 355(Pt 2), 297-305 (2001-04-04)
Subtraction hybridization after the exposure of keratinocytes to ultraviolet radiation identified a differentially expressed cDNA that encodes a protein of 630 amino acid residues possessing significant similarity to the catalytic domain of the sucrose-non-fermenting protein kinase (SNF1)/AMP-activated protein kinase (AMPK)
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