Materials

Figure 1.

Elution Order Ergocalcifero (Vitamin D2) and Cholecalciferol (Vitamin D3)

Conditions

Table 1.

Description

Table 2.

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Elution Order: Ergocalcifero (Vitamin D2) and Cholecalciferol (Vitamin D3)

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Small Molecule HPLC

HPLC Analysis of Vitamin D2 and D3 on Ascentis<sup>®</sup> Express C18

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This application demonstrates the suitability of the Ascentis Express C18, 5 μm, for the routine analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis Express C18, 5 μm are presented.

HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18