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HPLC Analysis of Tryptic Digest on BIOshell A160 Peptide C18

HPLC Analysis of Tryptic Digest on BIOshell A160 Peptide C18 application for HPLC

Matériaux

Colonne analytique

Réf. du produit
Description
Tarif

Ascentis® Express Peptide ES-C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

Étalon

Réf. du produit
Description
Tarif

Casein from bovine milk

suitable for substrate for protein kinase (after dephosphorylation), purified powder

CONDITIONS

column

BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)

mobile phase

[A] 0.1% formic acid in water; [B] 25:75, (0.4% formic acid in water):acetonitrile

gradient

0 to 70% B in 40 min.

flow rate

0.3 mL/min

column temp.

35 °C

detector

ESI(+), TOF

injection

2 μL

sample

10 pg/L in 0.1% formic acid

Description

Informations légales

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