HPLC Analysis of the Peptide Glycine-Leucine-Alanine Enantiomers on Astec^® CHIROBIOTIC^® TAG

column

CHIROBIOTIC TAG, 25 cm x 4.6 mm I.D., 5 μm particles (14024AST)

mobile phase

[A] 5 mM ammonium acetate, pH 4.1; [B] acetonitrile; (35:65, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 220 nm

injection

5 μL

sample

2 mg/mL in methanol