HPLC Analysis of Synthetic Food Dyes on Ascentis^® Express C18

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)

mobile phase

[A] 100 mM ammonium phosphate monobasic, pH 7.0 with ammonium hydroxide; [B] water; [C] acetonitrile

gradient

10% A constant; 85 to 25% B, 5 to 65% C in 6 min; 25% B, 65% C held for 5 min

flow rate

0.3 mL/min

column temp.

40 °C

detector

UV, 254 nm

injection

5 μL

sample

20 μg/mL each in water