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Merck

Peptides

Peptides application for HPLC

Matériaux

Application

Réf. du produit
Description
Tarif

Colonne HPLC Discovery® BIO Wide Pore C5

5 μm particle size, L × I.D. 15 cm × 4.6 mm

CONDITIONS

column

Discovery BIO Wide Pore C5, 15 cm × 4.6 mm I.D., 5 μm particles (568422-U)

mobile phase

[A] (95:5), 0.1% TFA in water:0.1% TFA acetonitrile; [B] (50:50), 0.1% TFA in water:0.1% TFA in acetonitrile

gradient

0-100% B in 65 min

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 215 nm

injection

50μL carboxymethylated apohemoglobin tryptic digest in 50 mM ammonium bicarbonate (568422-U)

Description

Informations légales

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany