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HPLC Analysis of Organic Acids on Ascentis® Express RP-Amide

HPLC Analysis of Organic Acids on Ascentis® Express RP-Amide application for HPLC

Matériaux

Colonne analytique

Réf. du produit
Description
Tarif

53913-U

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

Étalon

Réf. du produit
Description
Tarif

C0759

Acide citrique

99%

47910

Fumaric acid

≥99.0% (T)

T109

L-(+)-Tartaric acid

≥99.5%

147230

Acide acrylique

anhydrous, contains 200 ppm MEHQ as inhibitor, 99%

PHR1215

Lactic acid

Pharmaceutical Secondary Standard; Certified Reference Material

CONDITIONS

column

Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)

mobile phase

[A] 0.1% (v/v) TFA in [B] water

flow rate

0.3 mL/min

column temp.

35 °C

detector

UV, 210 nm

injection

1 μL

sample

0.2 - 4 g/L in mobile phase

Description

Remarque sur l'analyse

This application demonstrates the suitability of Ascentis Express RP-Amide for the analysis of organic acids under 100% aqueous conditions.

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS