HPLC Analysis of α-Methyl-α-Phenylsuccinimide Enantiomers on Astec^® CHIROBIOTIC^® TAG

column

CHIROBIOTIC TAG, 25 cm x 4.6 mm I.D., 5 μm particles (14024AST)

mobile phase

[A] hexane; [B] ethanol; (80:20, A:B)

flow rate

1 mL/min

column temp.

23 °C

detector

UV, 220 nm

injection

2 μL

sample

5 mg/mL in methanol