Les pyrazines dans le beurre de cacahouète
Matériaux
Colonne analytique
Fibre SPME
Ensemble de fibres de SPME, divinylbenzène/carboxène/polydiméthylsiloxane (DVB/CAR/PDMS)
50/30µm DVB/CAR/PDMS, StableFlex (1cm), needle size 24 ga, Manual Holder, pk of 3, plain gray hubProduit(s) apparenté(s)
Étalon
CONDITIONS
sample/matrix
5 g peanut butter in 40 mL vial
SPME fiber
divinylbenzene-Carboxen-polydimethylbenzene StableFlex (57328-U)
extraction
headspace, 30 min at 65 °C in heating block
desorption process
270 °C for 5 min
column
SUPELCOWAX 10, 30 m × 0.25 mm, 0.25 μm (24079)
oven
40 °C (5 min) to 230 °C at 4 °C/min
inj. temp.
270 °C
injection
splitless/split, closed 0.5 min, 270 °C, with 0.75 mm liner
detector
ion trap mass spectrometer, m/z =30-350 at 0.6 sec/scan. Selected ions used for quantitation.
carrier gas
Helium, 30 cm/sec
liner
0.75 mm
sample
peanut butter
Description
Remarque sur l'analyse
In this application, SPME was used for the analysis of volatiles in multifloral honey using a headspace method. Headspace SPME (HS-SPME) using a Carboxen/PDMS/DVB fiber coupled with gas chromatography-mass spectrometry (GC/MS) was used for the characterization of the volatile fraction of honey samples. Using SPME it was possible to obtain and distinguish the chemical fingerprints of different types of honey. The relatively polar SUPELCOWAX 10 GC column provides excellent separation of these polar compounds.
Informations légales
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