Increased intracellular pH is necessary for adult epithelial and embryonic stem cell differentiation.

Increased intracellular pH is necessary for adult epithelial and embryonic stem cell differentiation.

The Journal of cell biology (2016-11-09)

Bryne Ulmschneider, Bree K Grillo-Hill, Marimar Benitez, Dinara R Azimova, Diane L Barber, Todd G Nystul

RÉSUMÉ

Despite extensive knowledge about the transcriptional regulation of stem cell differentiation, less is known about the role of dynamic cytosolic cues. We report that an increase in intracellular pH (pHi) is necessary for the efficient differentiation of Drosophila adult follicle stem cells (FSCs) and mouse embryonic stem cells (mESCs). We show that pHi increases with differentiation from FSCs to prefollicle cells (pFCs) and follicle cells. Loss of the Drosophila Na

MATÉRIAUX

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Marque

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A7979

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Albumine de sérum bovin solution, 35% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

D5030

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Dulbecco′s Modified Eagle′s Medium, Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

A3085

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5-(N-Ethyl-N-isopropyl)amiloride

C3411

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2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein, ≥90% (HPLC)