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NPC mimics: probing the mechanism of nucleocytoplasmic transport.

Methods in cell biology (2014-05-27)
Tijana Jovanovic-Talisman, Brian T Chait, Michael P Rout
RÉSUMÉ

In vitro mimics of cellular machines have been recently engineered and utilized to investigate processes within cells. These devices can provide novel insights into biological mechanisms and have the potential to improve biotechnological processes such as separation. In particular, several devices have been designed to mimic translocation through nuclear pore complexes (NPCs). We describe here the fabrication of a biomimetic NPC using nanoporous filters lined with FG-repeats of proteins that create a selectivity barrier. We show the utility of this nanoselective filter as a testbed for the investigation of nucleocytoplasmic transport and demonstrate that this device closely reproduces key features of trafficking through the NPC.

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Sigma-Aldrich
Fluorure de phénylméthanesulfonyle, ≥99.0% (T)
Sigma-Aldrich
6-Aminocaproic acid, BioUltra, ≥99%