4X SDS Sample Loading Buffer for Western Blotting

This SDS sample loading buffer recipe is ideal for preparing and loading protein samples into gels for polyacrylamide gel electrophoresis analysis. This recipe calculator enables the accurate preparation of a 4X SDS sample loading buffer for any volume that you need. Input your desired volume, click the CALCULATE button, and the table will populate with the amounts of each component needed.

4X Solution concentrations:

• Tris-HCl: 0.2 M
• DTT: 0.4 M
• SDS: 277 mM, 8.0% (w/v)
• Bromophenol blue: 6 mM
• Glycerol: 4.3 M

OTHER RECIPE CALCULATORS FOR WESTERN BLOTTING BUFFERS & SOLUTIONS

Stock Solutions

• Tris-Buffered Saline (TBS) 10X Stock Solution
• 10X Phosphate-Buffered Saline (PBS)
• Stock Components for Western Blot Stripping Solutions

Wash & Blocking Buffers

• 1X Tris-Buffered Saline (TBS) Working Solution
• 1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST)
• 1X PBS (Phosphate-Buffered Saline) Concentration Recipe Calculator
• 1X Phosphate-Buffered Saline, 0.1% Tween® 20 Detergent (PBST)
• Non-Fat Dry Milk (NFDM) Blocking Buffer
• Bovine Serum Albumin (BSA) Blocking Buffer Recipe

Stripping Solutions

• Detergent-based Western Blot Stripping Buffer
• Acidic Western Blot Stripping Solution
• Stock Components for Western Blot Stripping Solutions