S7570

SGK2 Active human

≥75% (SDS-PAGE), aqueous glycerol solution, recombinant, expressed in E. coli

• Synonyme(s) : Serum/Glucocorticoid-Regulated Kinase 2
• Numéro MDL: MFCD07371188
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 200
• Pureté: ≥75% (SDS-PAGE)
• Forme: aqueous glycerol solution
• Activité spécifique: 100 units/mg protein (Bradford)
• Poids mol.: protein apparent mol wt ~66 kDa
• Numéro d'accès UniProt: Q9HBY8
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... SGK2(10110)

Description

Application

Kinase activity is measured as the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF), per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

Actions biochimiques/physiologiques

SGK2 is a member of the serum- and glucocorticoid-induced kinases (SGK) which are serine-threonine kinases and belong to the "AGC" kinase subfamily, which includes protein kinases A, G, and C and has SGK2 enhances Na⁺/K⁺-ATPase activity in a variety of cells. In addition, SGK2 mimics the function of SGK1 and SGK3 and participates in the regulation of renal epithelial Na⁺ channel ENaC activity.

Définition de l'unité

One unit will incorporate one nanomole of phosphate into the AKT/SGK substrate peptide(RPRAATF) per minute at 30 °C and pH 7.2 using a final concentration of 50 μM [32P] ATP.

Forme physique

Supplied at 50 μL in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable