Accéder au contenu
Merck
Toutes les photos(1)

Documents

RAB0010

Sigma-Aldrich

Angiotensin II EIA Kit

for serum, plasma, culture supernatant and cell lysates

Synonyme(s) :

Angiotensinogen, Serpin A8

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.32

Espèces réactives

mouse, human, rat

Conditionnement

kit of 96 wells (12 strips x 8 wells)

Technique(s)

ELISA: suitable
enzyme immunoassay: suitable

Entrée

sample type cell lysate
sample type culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 2.62 pg/mL
standard curve range: 0.1-1000 ng/mL

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... AGT(183)

Description générale

The Angiotensin II Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Angiotensin II peptide based on the principle of Competitive Enzyme Immunoassay. EIA-ANGII-1 can theoretically detect all active angiotensins, including ANGI, ANGII, ANGIII and ANGIV. However, it does not detect inactive angiotensinogen.

Immunogène

Angiotensin II, synthetic peptide

Application

Angiotensin II EIA Kit has been used for quantitative estimation of serum angiotensin-II.
For research use only. Not for use in diagnostic procedures.
Angiotensin II EIA Kit has been used for analyzing circulating and serum levels of angiotensin II (Ang II) in mice and rats respectively.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Actions biochimiques/physiologiques

Study on in vitro model shows that mechanical stretch induced Ang II release plays a vital role in mediating stretch-induced hypertrophic response. Ang II is implicated in maintaining blood pressure and has an important role in hypertension and in the pathophysiology of cardiovascular disorders. Ang II also has an essential role in growth induction, cell migration and mitosis of vascular smooth muscle cells. It enhances the production of collagen type I and III in fibroblasts, that results in thickening of the vascular wall and myocardium, and fibrosis. Variation in Ang II gene increases the risk of myocardial infarction, hypertension and left ventricular hypertrophy. Ang II expression is associated with heart failure, cardiac remodeling, diabetes, and the proliferative and inflammatory responses to arterial injury. The pharmacological agents such as angiotensin II-converting enzyme inhibitors or angiotensin II receptor antagonists can be used for treating Ang II-induced hypertension and congestive heart failure.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290

Conseils de prudence

P234 - P390

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Listes réglementaires

Les listes réglementaires sont principalement fournies pour les produits chimiques. Seules des informations limitées peuvent être fournies ici pour les produits non chimiques. L'absence d'indication signifie qu'aucun des composants n'est répertorié. Il incombe à l'utilisateur de s'assurer de l'utilisation sûre et légale du produit.

EU REACH Annex XVII (Restriction List)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Fernanda S Thomaz et al.
Journal of human hypertension, 35(2), 170-180 (2020-05-28)
The human gut microbiota plays a critical role in the regulation of adiposity, obesity and metabolic and cardiovascular disease. Wasabi is a pungent spice and its active component, allyl isothiocyanate, improves plasma triacylglycerol, cholesterol and high blood pressure in rodents
Emma N Bardsley et al.
Journal of molecular and cellular cardiology, 138, 234-243 (2019-12-15)
Chronically elevated angiotensin II is a widely-established contributor to hypertension and heart failure via its action on the kidneys and vasculature. It also augments the activity of peripheral sympathetic nerves through activation of presynaptic angiotensin II receptors, thus contributing to
Angiotensin II: a vasoactive hormone with ever-increasing biological roles.
Taubman MB
Circulation Research, 92(1), 9-11 (2003)
Daniela Volonte et al.
The Journal of biological chemistry, 296, 100242-100242 (2021-01-01)
Oncogenic K-Ras (K-RasG12V) promotes senescence in normal cells but fuels transformation of cancer cells after the senescence barrier is bypassed. The mechanisms regulating this pleiotropic function of K-Ras remain to be fully established and bear high pathological significance. We find
Autocrine release of angiotensin II mediates stretch-induced hypertrophy of cardiac myocytes in vitro.
Sadoshima J
Cell, 75(5), 977-984 (1993)
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique