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O5500

Sigma-Aldrich

Osmium tetroxide

Sealed ampule.

Synonyme(s) :

Osmium(VIII)-oxide, ‘Osmic acid’

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About This Item

Formule linéaire :
OsO4
Numéro CAS:
20816-12-0
Poids moléculaire :
254.23
Numéro CE :
244-058-7
Numéro MDL:
MFCD00011150
Code UNSPSC :
12171500
ID de substance PubChem :
57654490
Nomenclature NACRES :
NA.47

Densité de vapeur

8.8 (vs air)

Pression de vapeur

7 mmHg ( 20 °C)

Forme

powder

Pertinence de la réaction

reagent type: oxidant

Point d'ébullition

130 °C (lit.)

Pf

39.5-41 °C (lit.)

Chaîne SMILES 

O=[Os](=O)(=O)=O

InChI

1S/4O.Os

Clé InChI

VUVGYHUDAICLFK-UHFFFAOYSA-N

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Catégories apparentées

Description générale

Osmium tetroxide (OsO4) is a heavy tetrahedral, symmetrical molecule composed of osmium, a third-row transition metal. Osmium tetroxide is both a dye, and a non-coagulant fixative agent in electron microscopy primarily used for the examination of biological tissue components in the histopathology laboratory. Specimens are postfixed with osmium testroxide to ensure preservation of the lipids.

Application

Osmium tetroxide has been used as a fixative reagent for Brevibacterium mcbrellneri, and curcumin treated human lung cancer cell lines′ observation with a scanning electron microscope. It is also suitable as a fixative for ultrastructural analysis of Toxoplasma gondii′s skeletal muscle cells.

Actions biochimiques/physiologiques

Osmium tetroxide chemically combines with lipids making them insoluble; only 7% of the lipids can be extracted after the fixation. Therefore, the cell membranes comprising phospholipids become black and electron-dense after fixation in osmium tetroxide. It is also used to fix small amount of fat maintained in sections during paraffin processing.

Autres remarques

Due to the hazardous nature of osmium tetroxide, it is recommended to use an ampule opener when using this chemical.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Skull and crossbonesCorrosion
GHS06,GHS05

Mention d'avertissement

Danger

Mentions de danger

H300 + H310 + H330,H314

Classification des risques

Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Eye Dam. 1 - Skin Corr. 1B

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Certificats d'analyse (COA)

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