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MAK012

Sigma-Aldrich

Galactose Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonyme(s) :

Galactose Colorimetric/Fluorometric Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric or fluorometric tests

Application(s)

cosmetics
food and beverages

Méthode de détection

colorimetric
fluorometric

Maladie(s) pertinente(s)

pediatric diseases; neonatal diseases

Température de stockage

−20°C

Description générale

The new Galactose Assay Kit, MAK502, is now available! Galactose is a simple monosaccharide that serves as an energy source and as an essential component of glycolipids and glycoproteins. Galactose contributes to energy metabolism via its conversion to glucose by the enzymes that constitute the Leloir pathway. Defects in the genes encoding these proteins lead to the metabolic disorder galactosemia.

Application

  • Galactose assay kit has been used in the determination of reactor galactose concentration.
  • It has been used in D-galactose assay using the galactose oxidase enzymatic microreactor.
  • It has also been used in the general synthesis and characterization of O-(2-aminoethyl)-O′-galactosyl polyethylene glycol (H2N-PEG-GAL) derivative.

Adéquation

This kit is suitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.

Principe

In this assay kit, Galactose is oxidized resulting in a colorimetric (570 nm)/fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the galactose present. This kit has a linear range of detection between 0.2-1.0 nmole galactose for the fluorometric assay and 2-10 nmole galactose for the colorimetric assay.

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Réf. du produit
Description
Tarif

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Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H334,H412

Classification des risques

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

isorders of Carbohydrate Metabolism
David R, et al.
Emery and Rimoin's Principles and Practice of Medical Genetics (2013)
Joana Silva et al.
Journal of materials chemistry. B, 10(26), 5016-5027 (2022-06-21)
Oxime formation is a convenient one-step method for ligating reducing sugars to surfaces, producing a mixture of closed ring α- and β-anomers along with open-chain (E)- and (Z)-isomers. Here we show that despite existing as a mixture of isomers, N-acetylglucosamine
Galactosylated polyaspartamide copolymers for siRNA targeted delivery to hepatocellular carcinoma cells
Gennara C, et al.
International Journal of Pharmaceutics, 525(2), 397-406 (2017)
A system identification approach for developing model predictive controllers of antibody quality attributes in cell culture processes
Brandon D, et al.
Biotechnol. Progress, 33(6) (2017)
Rapid and simple preparation of thiol?ene emulsion-templated monoliths and their application as enzymatic microreactors
Josiane P L, et al.
Royal Society of Chemistry, 15, 2162-2172 (2015)
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Protocoles

Enzymatic Method for Determining Galactose (Galactose Assay)

This assay protocol is suitable for the colorimetric/fluorometric detection of Galactose and Lactose in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the Galactose Assay Kit.

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