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D2569

Sigma-Aldrich

Anti-DPPA5 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-Developmental pluripotency associated 5, Anti-ESG1, Anti-Embryonal stem cell-specific gene 1

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~14 kDa

Espèces réactives

mouse, human, rat

Concentration

~1 mg/mL

Technique(s)

immunohistochemistry: 1:100-1:250
western blot: 1:500-1:1,000

Numéro d'accès UniProt

A6NC42

Conditions d'expédition

dry ice

Température de stockage

−20°C

Informations sur le gène

human ... DPPA5(340168)
mouse ... Dppa5a(434423)
rat ... Dppa5(301101)

Description générale

The DPPA5 (developmental pluripotency associated 5) protein is strongly expressed in primordial germ cells, and the expression is down-regulated during germ cell development.

Immunogène

synthetic peptide corresponding to residues 43-60 human DPPA5. The sequence is 85% identical in mouse and rat.

Application

Anti-DPPA5 antibody produced in rabbit is suitable for immunohistochemistry at a working dilution of 1:100 to 1:250 and for immunoblotting at 1:500 to 1:1000.

Actions biochimiques/physiologiques

DPPA5 is specifically and differentially expressed in human cells that have pluripotency and can be used as a marker of pluripotent stem cells.

Forme physique

solution in phosphate buffered saline, containing 0.02% sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Soo-Kyoung Kim et al.
Stem cells (Dayton, Ohio), 23(4), 458-462 (2005-03-26)
Pluripotent embryonic germ cells (EGCs) can be derived from the culture of primordial germ cells (PGCs). However, there are no reports of gonocytes, following the stage of PGC development, becoming stem cell lines. To analyze the gene expression differences between
Shichao Liu et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 244(4), 619-627 (2015-01-27)
Mammalian first lineage segregation generates trophectoderm (TE) and pluripotent inner cell mass (ICM), which provides an ideal model for studying the mechanisms of maintenance and loss of pluripotency. In mouse, the transcription factor OCT4 restricts to ICM and plays a

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