IPI-2I

93100622, Porcine intestine, Epithelial-like

• Code UNSPSC : 41106514

Propriétés

• product name: IPI-2I, 93100622
• Source biologique: Porcine intestine
• Mode de croissance: Adherent
• Caryotype: Heteroploid
• Morphologie: Epithelial-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Boar Miniature male ileum, SV40 transformed

Description de la lignée cellulaire

Epitheloid cell line derived from the ileum of an adult histocompatible miniature male boar. Transformed by SV40 plasmid pSV3-neo. Electron microscopy analysis shows evidence of microvilli and desmosomes connecting cells. The cells were found to co-express cytokeratin 18 specific of simple luminal epithelial cells and vimentin associated with a mesenchymal origin. Susceptible to Transmissible Gastro-enteritis (TG) virus.

Application

Studies of epithelial cell differentiation.

Milieu de culture

DMEM + 4mM Glutamine + 0.024IU/ml Insulin + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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