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C1144

Sigma-Aldrich

Chlorazol Black

Powder

Synonyme(s) :

4-Amino-3-[[4′-[(2,4-diaminophenyl)azo][1,1′-biphenyl]-4-yl]azo]-5-hydroxy-6-(phenylazo)-2,7-naphthalenedisulfonic acid disodium salt, Chlorazole Black E, Direct Black 38

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About This Item

Formule empirique (notation de Hill):
C34H25N9Na2O7S2
Numéro CAS:
1937-37-7
Poids moléculaire :
781.73
Numéro C.I. (Colour Index):
30235
Numéro CE :
217-710-3
Numéro MDL:
MFCD00066363
Code UNSPSC :
12171500
ID de substance PubChem :
24892361
Nomenclature NACRES :
NA.47

product name

Chlorazol Black,

Forme

powder

Niveau de qualité

200

Composition

Dye content, ≥45% (based on Nitrogen)

Couleur

dark brown to black

Solubilité

H2O: 1 mg/mL

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

[Na+].[Na+].Nc1ccc(\N=N\c2ccc(cc2)-c3ccc(cc3)\N=N\c4c(N)c5c(O)c(\N=N\c6ccccc6)c(cc5cc4S([O-])(=O)=O)S([O-])(=O)=O)c(N)c1

InChI

1S/C34H27N9O7S2.2Na/c35-22-10-15-27(26(36)18-22)41-38-24-11-6-19(7-12-24)20-8-13-25(14-9-20)40-42-32-28(51(45,46)47)16-21-17-29(52(48,49)50)33(34(44)30(21)31(32)37)43-39-23-4-2-1-3-5-23;;/h1-18,44H,35-37H2,(H,45,46,47)(H,48,49,50);;/q;2*+1/p-2/b41-38+,42-40+,43-39+;;

Clé InChI

XRPLBRIHZGVJIC-UVHCWRHYSA-L

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Description générale

Chlorazol Black has strong affinity for cellulose and chitin. In plant tissues, it helps to differentiate between cellulose and lignin. It also helps in distinguishing fungal bodies from artefacts due to chitin staining. It is also used as an auxiliary stain for chromosomes.

Application

Chlorazol Black has been used to monitor the infection of mycorrhizal Allium porrum in plant roots. It has also been used for the staining of copepods.

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H319,H350,H361d

Classification des risques

Carc. 1B - Eye Irrit. 2 - Repr. 2

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges

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Certificats d'analyse (COA)

Lot/Batch Number
SHBM4400
MKCD3320
SLBR5011V
SLBN7918V
MKBQ9981V

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B Beije
Mutation research, 187(4), 227-234 (1987-04-01)
The genotoxic activity of the benzidine-derived azo dye, Direct Black 38 (DB38), was studied in vivo, using two different genetic end-points: unscheduled DNA synthesis in liver (UDS) and bone marrow micronucleus (MN). Exposure times were 12, 24 or 36 h.
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The N-doped TiO(2) photocatalyst was prepared by calcination of a hydrolysis product composed of titanium (IV) isopropoxide with ammonia as the precipitator. X-ray diffraction, surface area, XPS and UV-vis spectra analyses showed a nanosized anatase structure and the appearance of
Mustafa Işik et al.
Journal of hazardous materials, 114(1-3), 29-39 (2004-10-30)
An Upflow Anaerobic Sludge Blanket Reactor/Continuous Stirred Tank Reactor was used sequentially to decolorize and mineralize C.I. Direct Black 38 azo dye (3200 mg/L) in a synthetic wastewater containing glucose as co-substrate. At the steady state conditions color was effectively
Cell-wall-bound peroxidase activity in roots of mycorrhizal Allium porrum.
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The New phytologist, 109, 119-124 (1988)
Notes on Microscopical Technique for Zoologists null
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