NAD100-RO

β-Nicotinamide-adénine-dinucléotide hydrate

Grade I, free acid

• Synonyme(s) : β-DPN, β-NAD, Coenzyme 1, Cozymase, DPN, Diphosphopyridine nucléotide, NAD, Nadide
• Formule empirique (notation de Hill): C₂₁H₂₇N₇O₁₄P₂ · xH₂O
• Numéro CAS: 53-84-9
• Poids moléculaire : 663.43 (anhydrous basis)
• Numéro MDL: MFCD00036253
• Code UNSPSC : 12352200
• ID de substance PubChem : 329818699

Propriétés

• Pureté: 100% (dry weight)
• Forme: lyophilized
• Poids mol.: M_r 663.4
• Conditionnement: pkg of 1 g (10127965001); pkg of 5 g (10127973001)
• Fabricant/nom de marque: Roche
• Couleur: off-white
• pH: ~3.0 (50 mg/mL in water)
• λmax: 260 nm
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Chaîne SMILES: NC1=NC=NC2=C1N=CN2.O[C@@H]3[C@@H](COP(O)(OP(OC[C@@H](O4)[C@@H](O)[C@@H](O)[C@H]4[N+]5=CC=CC(C(N)=O)=C5)([O-])=O)=O)OC[C@@H]3O
• InChI: 1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1
• Clé InChI: BAWFJGJZGIEFAR-NNYOXOHSSA-N

Description

Description générale

Nicotinamide adenine dinucleotide (NAD) is a ubiquitously found electron carrier and a cofactor. NAD⁺ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD⁺ complexes, the enzyme-cofactor interactions are highly conserved.

Application

NAD (nicotinamide adenine dinucleotide), Grade I has been used in the kinetic studies of human class IV alcohol dehydrogenase.

NAD has been used in in vitro glycogen synthase kinase 3 (GSK3) deacetylation assay. It has also been used as a component in assay mix for the determination of kinetic characteristics using an enzyme-linked kinetic assay.

Actions biochimiques/physiologiques

NAD is also involved in microbial catabolism. β-NAD acts as a substrate for various enzymes in several cellular processes.

The NAD (nicotinamide adenine dinucleotide)/NADH ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo. It works as an electron acceptor.

Accepteur d'électrons

Conditionnement

Produit conditionné en fonction du poids solide.

Qualité

Purity: 100% β-NAD based on dry weight (enzymatically, CN-complex and absorbance); A₂₅₀nm - A₂₆₀nm : 0.83 ± 0.02, A₂₈₀nm - A₂₆₀nm : 0.22 ± 0.02
Absence of contaminants: LDH inhibitors are not detectable
Organic solvents: =0.05% (GC)

Caractéristiques

Absorbance coefficients: 17.6 x 10³l x mol^-1 x cm^-1 at 260nm, 5.9 x 10³l x mol^-1 x cm^-1 at 333nm (CN complex)

Notes préparatoires

Storage conditions (working solution): 10 mg/ml in aqueous solution, contains at least 99% NAD after 4 weeks at 2 to 8 °C

Autres remarques

Il s′agit de la forme courante du NAD.

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable