MABN748

Anti-TrpC3, clone 10H6 Antibody

clone 10H6, from mouse

• Synonyme(s) : Short transient receptor potential channel 3, TrpC3, Transient receptor protein 3, TRP-3, hTrp-3, hTrp3
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 10H6, monoclonal
• Espèces réactives: mouse
• Réactivité de l'espèce (prédite par homologie): human (based on 100% sequence homology)
• Technique(s): western blot: suitable
• Isotype: IgG1
• Numéro d'accès NCBI: NP_001124170
• Numéro d'accès UniProt: Q13507
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TRPC3(7222)

Description

Description générale

TrpC3, also known as Short transient receptor potential channel 3, transient receptor protein 3 or TRP-3, and encoded by the gene TRPC3/TRP3, forms a receptor activated calcium channel that is activated via ligand binding and transduces signals via the G-protein coupled tyrosine receptor kinases pathways.TrpC3 can also be activated by the release of internal calcium and is one of the principal receptors involved in maintaining Ca++ ion homeostasis in skeletal muscles and nervous tissue. TrpC3 is also a principal receptor involved in the Capacitative calcium entry (CCE) which is the mechanism behind Ca++ influx into cells that replenishes internal calcium stores. Additionally, TrpC3 receptor is important in axon guidance, phototransduction, and platelet activation. TrpC3 is localized to the cell membrane and consists of a multipass-though transport protein. TrpC3 is expressed principally in the brain but is also found in most other tissues and organs at lowered levels.

Immunogène

Recombinant protein corresponding to mouse TrpC3.

Application

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This Anti-TrpC3, clone 10H6 Antibody is validated for use in Western Blotting for the detection of TrpC3.

Western Blotting Analysis: A representative lot detected TrpC3 in cerebellar tissues of TRPC3 wild type mice and not TRPC3 knock out mice (Prof. Yizheng Wang, Shanghai Institutes for Biological Sciences (SIBS).
Western Blotting Analysis: A representative lot detected TrpC3 in rat liver and brain tissue lysates (Feng. S., et al. (2013). PNAS. 1-6).

Qualité

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TrpC3 in 10 µg of mouse brain tissue lysate.

Description de la cible

~110 kDa observed.

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable