MABF2225
Anti-HSV-2 VP16 Antibody, clone LP1
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
LP1, monoclonal
Poids mol.
calculated mol wt 54.6 kDa
observed mol wt ~37 kDa
Produit purifié par
using protein G
Espèces réactives
herpes simplex virus 1
Conditionnement
antibody small pack of 100 μL
Technique(s)
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG1
Séquence de l'épitope
Unknown
Conditions d'expédition
2-8°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
mouse ... vp16> VP16(1487335)
Catégories apparentées
Description générale
Alpha trans-inducing protein (UniProt: Q69113; also known as Alpha-TIF) is encoded by the VP16 (also known as UL48) gene (Gene ID: 1487335) in human Herpes Simplex virus 2. Herpes simplex virus (HSV) is a large enveloped DNA virus that replicates in the nuclei of mammalian cells. Its virion contains two regulatory proteins that are reported to modulate gene expression in infected cells: the transcriptional activator VP16 and the virion host shutoff protein vhs. VP16, an abundant structural protein, is shown to be essential for virion assembly and it stimulates transcription of the HSV immediate-early (IE) genes. Its transcriptional activation domain is localized in the C-terminal region. It is targeted to the TAATGARATTC consensus sequence found in IE promoters through interactions with the host factors Oct-1 and HCF. It is delivered by the infecting virion and acts during the earliest stages of infection to stimulate transcription of the viral IE genes, thereby facilitating the onset of the lytic program of viral gene expression. It is also reported to suppress vhs host protein synthesis and induce accelerated turnover of cellular and viral mRNAs. Clone LP1 is directed against VP16 in both HSV-1 and HSV-2. (Ref.: Smibert, CA., et al. (1994). J. Virol. 68(4); 2339-2346; McLean, C., et al. (1982). J. Gen. Virol. 63(2); 297-305).
Spécificité
Clone LP1 is a mouse monoclonal antibody that detects VP16 protein of Herpes simplex virus 2 (HSV-2) and displays cross reactivity with HSV-1.
Immunogène
Herpes simplex virus -2; strain 25766.
Application
Quality Control Testing
Evaluated by Western Blotting in lysate from HSV-1 infected Vero cells.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected VP16 protein in lysate from HSV-1 infected Vero cells.
Tested Applications
Immunoprecipitation Analysis): A representative lot immunoprecipitated HSV-2 VP16 in Immunoprecipitation applications (McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).
Western Blotting Analysis: A representative lot detected HSV-2 VP16 in Western Blotting applications (Crameri, M., et al. (2018). Nat Commun.; 9(1):1980; McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).
Immunofluorescence Analysis: A representative lot detected HSV-2 VP16 in Immunofluorescence applications (McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in lysate from HSV-1 infected Vero cells.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected VP16 protein in lysate from HSV-1 infected Vero cells.
Tested Applications
Immunoprecipitation Analysis): A representative lot immunoprecipitated HSV-2 VP16 in Immunoprecipitation applications (McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).
Western Blotting Analysis: A representative lot detected HSV-2 VP16 in Western Blotting applications (Crameri, M., et al. (2018). Nat Commun.; 9(1):1980; McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).
Immunofluorescence Analysis: A representative lot detected HSV-2 VP16 in Immunofluorescence applications (McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-HSV-2 VP16, clone LP1, Cat. No. MABF2225, is a mouse monoclonal antibody that detects Alpha trans-inducing protein (VP16) and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Forme physique
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique