MAB353B

Anti-Nestin Antibody, clone rat-401, Biotin Conjugate

clone rat-401, from mouse, biotin conjugate

• Synonyme(s) : rat401, NES, Nbla00170, FLJ21841
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: biotin conjugate
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: rat-401, monoclonal
• Espèces réactives: mouse, rat
• Ne doit pas réagir avec: human
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable
• Isotype: IgG1
• Numéro d'accès NCBI: NP_037119
• Numéro d'accès UniProt: P21263
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... NES(10763) ; mouse ... Nes(18008) ; rat ... Nes(25491)

Description

Description générale

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.

Spécificité

This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.

Immunogène

Embryonic rat spinal cord

Application

Detect Nestin using this mouse monoclonal antibody, Anti-Nestin Antibody, clone rat-401, Biotin Conjugate validated for use in ICC & IHC.

Evaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022).
Immunocytochemsitry Analysis: A 1:200 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).
Evaluated by Immunocytochemistry in ReNcell VM human neural progenitor cells (SCC008).
Immunocytochemsitry Analysis: A 1:200 dilution of this antibody did not detect Nestin in ReNcell VM human neural progenitor cells (SCC008). Evaluated by Immunohistochemistry in rat hindbrain brain tissue.
Immunohistochemsitry Analysis: A 1:50 dilution of this antibody detected Nestin in rat hindbrain brain tissue.

Research Category
Stem Cell Research

Neuroscience

Research Sub Category
Developmental Neuroscience

Neural Stem Cells

Qualité

Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:200 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).

Description de la cible

200-220 kDa calculated

Forme physique

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15mg/ml BSA.

Stockage et stabilité

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Remarque sur l'analyse

Control
Mouse cortical neural stem cells (SCR029)

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable