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Merck
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Key Documents

MAB2022

Sigma-Aldrich

Anti-Keratan Sulfate Antibody, clone EFG-11

ascites fluid, clone EFG-11, Chemicon®

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

ascites fluid

Clone

EFG-11, monoclonal

Espèces réactives

sheep, bovine, pig, canine, mouse, human

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
radioimmunoassay: suitable
western blot: suitable

Isotype

IgG2b

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... LUM(4060)

Spécificité

Recognizes 2-3 disaccharide units on both skeletal (type II) and corneal (type I) keratan sulfate chains either in isolated or proteoglycan-bound form. The epitope is resistant to proteolytic or alkaline cleavages and partially sensitive to keratanase or other endo-B-galactosidase treatment.

Immunogène

Human articular cartilage proteoglycan digested with chondroitinase ABC.

Application

Anti-Keratan Sulfate Antibody, clone EFG-11 is an antibody against Keratan Sulfate for use in ELISA, RIA & WB.
Research Category
Cell Structure
Research Sub Category
Cytokeratins
Western blot at 1:1000 - 1:4000

ELISA at 1:5,000-1:20,000

RIA at 1:30,000-1:500,000

May be used in a competitive inhibition assay to measure keratan sulfate levels.

Forme physique

Ascites liquid, no preservative.

Stockage et stabilité

Maintain at -20°C in convenient aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

E J Thonar et al.
Arthritis and rheumatism, 28(12), 1367-1376 (1985-12-01)
We have developed an enzyme-linked immunosorbent-inhibition assay which makes use of a monoclonal antibody specific for keratan sulfate to quantify keratan sulfate present as single chains in adult human serum. In adults hospitalized with conditions not thought to affect the
Proteoglycan epitopes as potential markers of normal and pathologic cartilage metabolism.
V C Hascall et al.
Arthritis and rheumatism, 30(5), 586-588 (1987-05-01)
Respiratory syncytial virus infection of human airway epithelial cells is polarized, specific to ciliated cells, and without obvious cytopathology.
Liqun Zhang, Mark E Peeples, Richard C Boucher, Peter L Collins, Raymond J Pickles et al.
Journal of virology null
Transplantation of mesenchymal stem cells in a canine disc degeneration model.
Akihiko Hiyama,Joji Mochida,Toru Iwashina,Hiroko Omi,Takuya Watanabe,Kenji Serigano et al.
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society null
Liping Yang et al.
Journal of the Association for Research in Otolaryngology : JARO, 19(4), 353-362 (2018-04-25)
Otoconia-related vertigo and balance deficits, particularly benign paroxysmal positional vertigo (BPPV), are common. Our recent studies in humans show that, while BPPV prevalence greatly increases with age in both genders, peri-menopausal women are especially susceptible. In the present study, we

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