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HSP3MAG-63K

Millipore

MILLIPLEX® Human Sepsis Magnetic Bead Panel 3 - Immune Response Multiplex Assay

Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple sepsis biomarkers in human serum, plasma and cell culture samples.

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About This Item

Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.84

Niveau de qualité

200

Espèces réactives

human

Fabricant/nom de marque

Milliplex®

assay range

accuracy: 82%
(Lactoferrin)
accuracy: 98%
(Lipocalin-2/NGAL)
standard curve range: 10-10,000 pg/mL
(Resistin)
standard curve range: 195-200,000 pg/mL
(Lactoferrin and Thrombospondin-1 (TSP1))
standard curve range: 20-20,000 pg/mL
(Neutrophil Elastase-2 (ELA2))
standard curve range: 29-30,000 pg/mL
(Lipocalcin-2/NGAL)

Technique(s)

multiplexing: suitable

Méthode de détection

fluorometric (Luminex xMAP)

Conditions d'expédition

wet ice

Description générale

Sepsis or SIRS (systemic inflammatory response syndrome) results from infection, whether bacterial, viral, fungal, or parasitic. According to the CDC, sepsis is the second leading cause of death in non-coronary ICU patients in the U.S. The pathogenesis of sepsis is very complex. Both an innate and acquired immune response to infection interrupts homeostasis with a shift of cytokine expression that increases immunosuppression. In addition, migration of the leukocytes in the inflamed tissues secretes a tissue factor leading to the formation of thrombin, also triggering the release of chemokines and adhesion molecules through endothelial cells. Severe sepsis occurs when hypotension or hypoperfusion to one or more organs leads to organ dysfunction, which, in turn, can cause septic shock, multiple organ dysfunction syndrome (MODS) and death.

MILLIPLEX® Human Sepsis Panel 3 is to be used for the simultaneous quantification of any or all of the following analytes in human serum, plasma and cell / tissue culture supernatant samples: Lactotransferrin (LTF), Neutrophil Elastase-2 (ELA2), NGAL/Lipocalin-2, Resistin, Thrombospondin-1. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Immune Response

Spécificité

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: Lactotransferrin, Lipocalin-2/NGAL, Neutrophil Elastase-2 (ELA2), Resistin, Thrombospondin-1 (TSP-1)
  • Recommended Sample type: Serum, plasma, or cell culture supernatant
  • Recommended Sample dilution: 1:100 serum/plasma
  • Assay Run Time: One day
  • Research Category: Inflammation & Immunology

Caractéristiques et avantages

Design your multiplex kit by choosing available analytes within this panel.

Autres remarques

Sensitivity: Refer to kit protocol for individual sensitivites.

Informations légales

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Organes cibles

Respiratory Tract

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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Atqah AbdulWahab et al.
Cureus, 13(6), e15948-e15948 (2021-07-06)
Background and purpose Neutrophil elastase (NE) has been implicated in the pathogenesis of airway inflammation in cystic fibrosis (CF) patients and it impairs defenses against Pseudomonas aeruginosa (PA) infection or colonization. Sputum NE may act as a biomarker of neutrophilic inflammation in CF

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