AB3075
Anti-Aquaporin 7 Antibody
Chemicon®, from rabbit
Synonyme(s) :
AQP7
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
rat
Fabricant/nom de marque
Chemicon®
Technique(s)
ELISA: suitable
western blot: suitable
Numéro d'accès NCBI
1 of 4
Cet article | 8.18392 | 8.01113 | 8.01111 |
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form solid | form powder | form crystals | form crystals |
| storage temp. 2-30°C | storage temp. 2-30°C | storage temp. 2-30°C | storage temp. 2-30°C |
| mp 115-118 °C | mp 217-220 °C | mp 55-58 °C | mp 157-160 °C |
| bp 159 °C/13 hPa | bp - | bp 209-211 °C/1013 hPa | bp 274 °C/1013 hPa |
Description générale
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26 kDa), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Aquaporin-7 (rAQP7, 269aa) is abundantly expressed in testis. It is involved in water, glycerol and urea transport. Aquaporin-7 is also found in adipose tissue, kidney, and heart. Aquaporin-7 has been localized to in the spermatids and at maturing sperms. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.
Spécificité
Aquaporin 7. The immunogen peptide is unique to Aquaporin-7 without significant homology to any other Aquaporins.
The peptide has 81% similarity with the mouse Aquaporin-7
Immunogène
An 18 AA synthetic peptide within the N-terminal domain of rat Aquaporin-7 (Ishibashi et al. 1997) was selected for antibody production. This domain is predicted to be cytoplasmic.
Application
Detect Aquaporin 7 using this Anti-Aquaporin 7 Antibody validated for use in ELISA & WB.
Note: We recommend the use of 0.5-1.0% milk in all primary/secondary antibody incubations to suppress non-specific reactivity.
Western blot: 1-10 μg/mL using Chemiluminescence technique
ELISA: 0.5-1.0 μg/mL when tested against 1 μg/mL of the immunogen peptide.
Immunohistochemistry: We recommend using the affinity purified antibody at 2-10 μg/mL in paraformaldehyde fixed sections of tissues.
Optimal working dilutions must be determined by end user.
Western blot: 1-10 μg/mL using Chemiluminescence technique
ELISA: 0.5-1.0 μg/mL when tested against 1 μg/mL of the immunogen peptide.
Immunohistochemistry: We recommend using the affinity purified antibody at 2-10 μg/mL in paraformaldehyde fixed sections of tissues.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Forme physique
Affinity Purified 1 mg/ml soln in PBS, pH 7.5 and 0.1% BSA and 0.05% sodium azide
Stockage et stabilité
Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Aquaporin expression is downregulated in a murine model of colitis and in patients with ulcerative colitis, Crohn's disease and infectious colitis.
J A Hardin, L E Wallace, J F K Wong,
Cell and Tissue Research null
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
