07-421-I

Anti-dimethyl Histone H3 (Lys27)

from chicken, purified by affinity chromatography

• Synonyme(s) : H3K27me2, H3.3B, Lys27 dimethylated, H3 histone, family 3A, Lys27 dimethylated, H3 histone, family 3B, Lys27 dimethylated, Histone H3, Lys27 dimethylated
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: chicken
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: rat, mouse, chicken, human
• Ne doit pas réagir avec: yeast
• Technique(s): ChIP: suitable; dot blot: suitable; western blot: suitable
• Isotype: IgY
• Numéro d'accès NCBI: NP_002098
• Numéro d'accès UniProt: P84243
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: dimethylation (Lys27)
• Informations sur le gène: human ... HIST1H3F(8968)

Description

Description générale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

Spécificité

Broad species cross-reactivity expected based on sequence homology.

Specificity of this chicken polyclonal antibody toward H3K27me2 modification has been confirmed using AbSurance H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665) and AbSurance H3 Antibody Specificity Array (Cat. No. 16-667). No cross-reactivity toward histone H3 peptides with unmodified K27 and other K27 modifications, H2A, H2B, or H4 peptides.

Immunogène

KLH-conjugated peptide corresponding to amino acids 22-32 of human histone H3 with dimethylated Lys27.

Application

Anti-dimethyl Histone H3 (Lys27) Antibody, Cat. No. 07-421-I, is a highly specific chicken polyclonal antibody that targets histone H3 Lys27 dimethylation (H3K27me2) and has been tested in Dot Blot, Chromatin Immunoprecipitation, and Western Blotting.

Chromatin Immunoprecipitation Analysis: 12 µL from a representative lot detected Lys27-dimethylated histone H3 occupancy at the -globin promoter region using HeLa nuclear extract (0.1 million cell eq. per ChIP) and Magna ChIP HiSens Chromatin Immunoprecipitation Kit (Cat. No. 17-10460).

Dot Blot Analysis: A 1:500 dilution from a representative lot specifically detected histone H3 peptide with K27me2 modification, but not H3 peptides with unmodified K27 and other K27 modifications, H2A, H2B, or H4 peptides by dot blot analysis using AbSurance H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665) and AbSurance H3 Antibody Specificity Array (Cat. No. 16-667).

Research Category
Epigenetics & Nuclear Function

Qualité

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Lys27-dimethylated histone H3 in 10 µg of HeLa acid extract.

Description de la cible

~17 kDa observed. 15.20 kDa calculated (based on UniProt P84243 with Met1 removed). Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity purified.

Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1