LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis® Express OH5
Conditions | |
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column | Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53757-U) |
column temp. | 30 °C |
mobile phase | [A] water with 0.1% formic acid/10 mM ammonium formate; [B] acetonitrile with 0.1% formic acid/10 mM ammonium formate; (A:B, 15:85) |
flow rate | 0.2 mL/min |
pressure | 1150 psi (79 bar) |
sample | 10 ng/mL in mobile phase |
injection | 1 µL |
detector | MS, ESI(+), MRM mode |
Description | |
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Analysis Note | The present work establishes an HILIC LC/MS/MS method for analysis of water soluble vitamins B1 and B6 on an Ascentis® Express OH5 column. LC/MS grade solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. |
Categories | Analytical Chromatography, Immunosuppressants |
Featured Industry | Vitamins, Nutraceuticals, and Natural Products |
suitability | application for HPLC |
Materials
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